Reporter

Part:BBa_K1088026:Design

Designed by: Patrick Rosendahl Andreassen   Group: iGEM13_SDU-Denmark   (2013-09-19)


B. subtilis dxs-GFP protein fusion (lac promoter with lac inhibitor: IPTG inducible)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
    Illegal PstI site found at 2518
    Illegal PstI site found at 2964
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 2518
    Illegal PstI site found at 2964
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
    Illegal PstI site found at 2518
    Illegal PstI site found at 2964
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
    Illegal PstI site found at 2518
    Illegal PstI site found at 2964
    Illegal NgoMIV site found at 2417
    Illegal AgeI site found at 2310
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2259
    Illegal BsaI.rc site found at 3973
    Illegal SapI.rc site found at 2958


Design Notes

A linker was placed between the two genes to decrease the risk of misfolding and loss of function.


Source

The components specify the source

References